CRAMPON marc (BRGM, Orléans, FRANCE)

First Name: Marc

Name: Crampon

Position: Researcher

Email: m.crampon@brgm.fr

Institute / University / Company: BRGM

Research Unit: Geomicrobiology and Environmental Monitoring

Address (city, state): 3 avenue Claude Guillemin 45100 ORLEANS

Country: France

Please give 5 key-words showing your research / interest in Microbial Ecotoxicolgy:

  • Persistant Organic Pollutants
  • Bioremediation
  • PAHs
  • Contaminants ecotoxicity
  • Bioavailability

Main kinds of contaminant(s) of interest:

  • Polycyclic Aromatic Hydrocarbons (PAHs)
  • chlorinated hydrocarbons (PCE and metabolites)
  • BTEX
  • pesticides (chlordecone)
  • Nanoparticles

Main kinds of microorganisms of interest:

  • Natural bacterial community in soils and groundwater
  • biosurfactant-producing bacteria
  • pollutant-degrading bacteria and fungi.

Main kinds of ecosystems of interest:

  • Contaminated soils
  • groundwater and sediments.

Do you give courses on Microbial Ecotoxicology ?: NO

Most relevant articles in the field of Microbial Ecotoxicology (max.5):

  • Crampon, M., Bodilis, J., and Portet-Koltalo, F. (2018a). Linking initial soil bacterial diversity and polycyclic aromatic hydrocarbons (PAHs) degradation potential. Journal of Hazardous Materials 359, 500-509. doi: https://doi.org/10.1016/j.jhazmat.2018.07.088.
  • Crampon, M., Bureau, F., Akpa-Vinceslas, M., Bodilis, J., Machour, N., Le Derf, F., et al. (2014). Correlations between PAH bioavailability, degrading bacteria, and soil characteristics during PAH biodegradation in five diffusely contaminated dissimilar soils. Environmental Science and Pollution Research 21(13), 8133-8145. doi: 10.1007/s11356-014-2799-6.
  • Crampon, M., Cébron, A., Portet-Koltalo, F., Uroz, S., Le Derf, F., and Bodilis, J. (2017). Low effect of phenanthrene bioaccessibility on its biodegradation in diffusely contaminated soil. Environmental Pollution 225, 663-673. doi: https://doi.org/10.1016/j.envpol.2017.03.053.
  • Crampon, M., Hellal, J., Mouvet, C., Wille, G., Michel, C., Wiener, A., et al. (2018b). Do natural biofilm impact nZVI mobility and interactions with porous media? A column study. Science of The Total Environment 610–611, 709-719. doi: https://doi.org/10.1016/j.scitotenv.2017.08.106.
  • Wille, G., Hellal, J., Ollivier, P., Richard, A., Burel, A., Jolly, L., et al. (2017). Cryo-Scanning Electron Microscopy (SEM) and Scanning Transmission Electron Microscopy (STEM)-in-SEM for Bio- and Organo-Mineral Interface Characterization in the Environment. Microscopy and Microanalysis 23(6), 1159-1172. doi: 10.1017/S143192761701265X.

EcotoxicoMic Webinar: Valentin DUPRAZ (IFREMER-University of Nantes, France)

4th EcotoxicoMic Webinar

Friday, October 12, 2018  (9:10 am, Paris Time Zone)

Webinar Registration: click here


Valentin DUPRAZ

(IFREMER-University of Nantes, France)

dupraz

How to interpret the toxicity of complex environmetal mixtures? a method combining analytical chemistry and mixture modelling approaches

Valentin Dupraz1,2, Dominique Ménard1, Nathalie Tapie3,4, Farida Akcha1, Hélène Budzinski3,4, Sabine Stachowski-Haberkorn1
1 Ifremer, Laboratoire d’Écotoxicologie, rue de l’île d’Yeu, BP 21105, 44311 Nantes cedex 03, France; 2 Université de Nantes, 2, rue de la Houssinière, BP 92208, 44322 Nantes Cedex 03, France; 3 Université de Bordeaux, UMR 5805, EPOC, Laboratoire de Physico Toxico Chimie de l’environnement, 351 Cours de la Libération, CS 10004, F-33405 Talence Cedex, France; 4 CNRS, UMR 5805, EPOC, Laboratoire de Physico Toxico Chimie de l’environnement, 351 Cours de la Libération, CS 10004, F-33405 Talence Cedex, France

Keywords: microalgae; passive samplers; synergy; mixtures; vineyards

Pesticides used in viticulture end up in aquatic systems downstream of vineyard plots.  Concentrations of single substances are often below the maximum residue level imposed by the WFD but few is known about their potential toxicity in mixtures.

According to their chemical properties, pesticides accumulate over time in POCIS passive samplers deployed in contaminated environments and the toxicity of the whole mixture from POCIS can be tested after extraction. As phytoplankton plays a key role in the aquatic food web, this study aimed to assess the toxicity of pesticides as well as POCIS extracts towards the growth of two marine microalgae, Tisochrysis lutea (haptophyte) and Skeletonema marinoi (diatom). POCIS were placed in three stations along a contamination gradient in small watercourses close to the vineyards plots over four one-month periods.

The toxicity tests consisted in 96-h exposures of microalgae to ii) single and binary combinations of pesticides expected in the extracts and ii) POCIS extracts, in sterile 48-well microplates incubated under controlled conditions. Each species was exposed to six concentrations of the single or mixed (at three different mixture ratios) substances and five dilutions of the POCIS extracts. The toxicity was assessed by calculating the growth rate inhibition using chlorophyll fluorescence. To assess the interactive effect of pesticide mixtures, a mixture modelling approach was used. Chemical analyses were performed to monitor exposure concentrations of the tested pesticides in the microplate test system and to quantify the expected substances in diluted POCIS extracts.

Nine pesticides tested singly induced a significant toxicity. The binary combination of isoproturon and metazchlor was found to be significantly synergistic for the diatom S. marinoi. POCIS extracts induced a varying toxicity depending on the period and/or station. The toxicity of the extracts was partially explained by compiling results from both single and mixed substances toxicity assays and chemical analyses of the diluted POCIS extracts.

The testing of complex mixtures from the environment provides additional information regarding the contamination of aquatic ecosystems and enlights the necessity of focusing on mixture effects, along with single compound toxicity.

FERNANDES Isabel (University of Minho, Braga, PORTUGAL)

Fernandes

First Name: Isabel

Name: Fernandes

Position: PostDoc

Email: isabelrodriguesfernandes@gmail.com

Institute / University / Company: University of Minho

Research Unit: Centre of Molecular and Environmental Biology (CBMA)

Address (city, state): Campus de Gualtar, 4710-057 Braga

Country: Portugal

Please give 5 key-words showing your research / interest in Microbial Ecotoxicolgy:

  • leaf decomposition
  • aquatic fungi
  • temperature
  • biodiversity and ecosystem functioning
  • freshwaters

Main kinds of contaminant(s) of interest:

  • nutrients
  • fungicides

Main kinds of microorganisms of interest:

  • aquatic fungi
  • bacteria

Main kinds of ecosystems of interest:

  • freshwaters

Do you give courses on Microbial Ecotoxicology ?: NO

Most relevant articles in the field of Microbial Ecotoxicology (max.5):